Building on over 25 years experience in designing and manufacturing femtosecond lasers and wavelength-tunable sources, LIGHT CONVERSION has recently introduced CRONUS-2P and CRONUS-3P microscopy-dedicated femtosecond laser sources covering applications in functional neuroimaging, optogenetics, and deep imaging using medium-repetition-rate three-photon excitation and fast high-repetition-rate two-photon imaging.
CRONUS-3P provides μJ-level pulses down to 50 fs at repetition rates of up to 2 MHz and tunable in the short-wavelength infrared (SWIR) range from 1.25 to 1.8 μm, thus covering the biological transparency windows at 1.3 μm and 1.7 μm for three-photon microscopy. Typically, multiphoton imaging in the SWIR range requires a complex multi-device laser system and, usually, a large part of an optical table and skilled staff, a reality that encumbers neuroscience and other biomedical applications. CRONUS-3P system is a next-generation, industrial-grade, single-supplier solution that is more compact, more reliable, and more versatile, and achieves tunable femtosecond excitation with an integrated group delay dispersion (GDD) control, ensuring optimal pulse duration at the sample, and industrial-grade design to guarantee excellent short- and long-term output stability.
CRONUS-2P is a femtosecond laser providing three simultaneous synchronized outputs with high repetition rate, high output power, short pulse duration, and GDD control, making it the ultimate source for nonlinear microscopy. Two outputs are independently tunable in the 680 – 960 nm and 960 – 1300 nm ranges, while the third is fixed at 1025 nm. The CRONUS-2P can be used for simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima, whereas second- and third-harmonic emission (SHG and THG) can be spectrally shifted for ease of detection or resonant enhancement. The three simultaneous outputs also provide for advanced coherent anti-Stokes and stimulated Raman scattering (CARS and SRS) applications with dual-band imaging, a broader selection of vibrational resonance frequencies, constant-difference dual-beam tuning, resonant enhancement, and more.
